Ewing’s Sarcoma as a Second Malignancy in Long-Term Survivors of Childhood Hematologic Malignancies

Figure 1

(a) Biopsy of the cervical mass of patient 1, 3 years prior to current presentation. Diffuse infiltration of the lymph node tissue with effacement of the lymph node architecture and spread to the adjacent adipose tissue (star) by sheets of atypical lymphatic blasts (inset) (H&E; original magnification 400x). (b) Core biopsy of the paravertebral mass of patient 1. Solid small, blue round undifferentiated tumor with strong immunohistochemical membranous expression of CD99 (brown reaction product shown in the inset) (H&E; original magnification 400x). (c) Sanger-sequencing of the RT-PCR amplicon derived from the fusion transcript reveals the most common EWSR1-FLI1 fusion of exon 7 to exon 6, respectively, in the paravertebral tumor tissue of patient 1 confirming the diagnosis of a Ewing sarcoma.

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