Using the Spleen as an In Vivo Systemic Immune Barometer Alongside Osteosarcoma Disease Progression and Immunotherapy with α-PD-L1

Table 6

Population distribution comparison of MLCs isolated from the spleens of mice from TF tumor-bearing (n = 5) and SH (n = 4) groups.

Population	TF % expression	SH % expression	p
MLCs	5.27 ± 1.11%	1.39 ± 0.4%	0.00031
Monocytes/macrophages	4.92 ± 1.29%	7.49 ± 1.7%	0.036
Granulocytes	57.49 ± 9.62%	74.69 ± 4.55	0.014
Tgm2^+ macrophages	18.58 ± 5.69%	4.22 ± 2.66%	0.0025
Arg1^+ macrophages	0.3 ± 0.56%	0 ± 0%	0.32
Cxcl9^+ macrophages	18.28 ± 5.38%	1.44 ± 1.23%	0.00052
Nos2^+ macrophages	97.99 ± 1.62%	16.56 ± 10.19%	4.2E-07

The statistics for each group are summarized accordingly with significance being assigned to those populations presenting values < 0.00714 from a two-tailed Student’s T-test with Bonferroni correction and 95% confidence as denoted by an asterisk. MLC = myeloid lineage cell; TF = transfected; Tgm2 = transglutaminase 2; Arg1 = arginase 1; Cxcl9 = chemokine (C-X-C) motif ligand 9; Nos2 = nitric oxide synthase 2.