Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration

Figure 5

Effect of Plexin-B2 siRNA knockdown on cell proliferation. Cell growth assays were performed at 24 hr, 48 hr, and 72 hr after siRNA treatment in Rh30, Rh41, CF-1, RD, and Rh18. Plexin-B2 siRNA significantly reduced cell proliferation at 48 hr and 72 hr in Rh30 and Rh41 cell lines. Similarly, in eRMS cell lines, RD and Rh18, cell proliferation decreased significantly as early as 24 hr and persisted through 48 hr and 72 hr time points. However, in CF-1, cell proliferation significantly increased at all time points. (The data on graphs show means ± SEM. , , ).