Research Article
Plexin-B2 and Semaphorins Do Not Drive Rhabdomyosarcoma Proliferation or Migration
Table 1
IHC analyses of Plexin-B2, SEMA4C, SEMA4D, and SEMA4F using TMA for RMS.
| | | Plexin-B2 (Homo sapiens) | | Plexin-B2 (Mus musculus) | | aRMS | eRMS | aRMS | eRMS | UPS | | # of samples | % of aRMS | # of samples | % of eRMS | # of samples | % of aRMS | # of samples | % of eRMS | # of samples | % of UPS | | Stain present | 1 of 1 | 100% | 3 of 3 | 100% | Stain present | 12 of 16 | 75% | 6 of 7 | 86% | 17 of 33 | 52% | | % positive (tumor cells): | | | | | % positive (tumor cells): | | | | | | | | 0 | 0 | 0% | 0 | 0% | 0 | 0 | 4% | 0 | 0% | 0 | 0% | | 1–10% | 0 | 0% | 0 | 0% | 1–10% | 0 | 0% | 0 | 0% | 0 | 0% | | 11–50% | 0 | 0% | 0 | 0% | 11–50% | 0 | 0% | 0 | 0% | 3 | 18% | | 51–80% | 0 | 0% | 0 | 0% | 51–80% | 0 | 0% | 1 | 17% | 1 | 6% | | 81–100% | 1 | 100% | 3 | 100% | 81–100% | 12 | 100% | 5 | 83% | 13 | 76% | | Stain intensity (tumor cells): | | | | | Stain intensity (tumor cells): | | | | | | | | None | 0 | 0% | 0 | 0% | None | 0 | 0% | 0 | 0% | 0 | 0% | | Weak | 1 | 100% | 0 | 0% | Weak | 1 | 8% | 1 | 17% | 5 | 29% | | Intermediate | 0 | 0% | 1 | 33% | Intermediate | 0 | 0% | 0 | 0% | 6 | 35% | | Strong | 0 | 0% | 2 | 67% | Strong | 11 | 92% | 5 | 83% | 6 | 35% |
| | | SEMA4C (Homo sapiens) | | SEMA4C (Mus musculus) | | | aRMS | eRMS | | aRMS | eRMS | UPS | | | # of samples | % of aRMS | # of samples | % of eRMS | | # of samples | % of aRMS | # of samples | % of eRMS | # of samples | % of UPS | | Stain present | 1 of 1 | 100% | 3 of 3 | 100% | Stain present | 13 of 16 | 81% | 6 of 7 | 86% | 20 of 33 | 61% | | % positive (tumor cells): | | | | | % positive (tumor cells): | | | | | | | | 0 | 1 | 100% | 1 | 33% | 0 | 1 | 8% | 1 | 17% | 5 | 25% | | 1–10% | 0 | 0% | 2 | 67% | 1–10% | 1 | 8% | 1 | 17% | 4 | 20% | | 11–50% | 0 | 0% | 0 | 0% | 11–50% | 7 | 54% | 2 | 33% | 7 | 35% | | 51–80% | 0 | 0% | 0 | 0% | 51–80% | 3 | 23% | 1 | 17% | 2 | 10% | | 81–100% | 0 | 0% | 0 | 0% | 81–100% | 1 | 8% | 1 | 17% | 2 | 10% | | Stain intensity (tumor cells): | | | | | Stain intensity (tumor cells): | | | | | | | | None | 1 | 100% | 1 | 33% | None | 1 | 21% | 1 | 17% | 5 | 25% | | Weak | 0 | 0% | 2 | 67% | Weak | 7 | 62% | 4 | 67% | 10 | 50% | | Intermediate | 0 | 0% | 0 | 0% | Intermediate | 4 | 14% | 1 | 17% | 1 | 5% | | Strong | 0 | 0% | 0 | 0% | Strong | 1 | 3% | 0 | 0% | 4 | 20% |
| | | SEMA4D (Homo sapiens) | | SEMA4D (Mus musculus) | | Stain present | 1 of 1 | 100% | 3 of 3 | 100% | Stain present | 14 of 16 | 88% | 6 of 7 | 86% | 19 of 32 | 59% | | | aRMS | eRMS | | aRMS | eRMS | UPS | | | # of samples | % of aRMS | # of samples | % of eRMS | | # of samples | % of aRMS | # of samples | % of eRMS | # of samples | % of UPS | | % positive (tumor cells): | | | | | % positive (tumor cells): | | | | | | | | 0 | 0 | 0% | 0 | 0% | 0 | 0 | 0% | 0 | 0% | 0 | 0% | | 1–10% | 0 | 0% | 0 | 0% | 1–10% | 0 | 0% | 0 | 0% | 1 | 5% | | 11–50% | 0 | 0% | 0 | 0% | 11–50% | 0 | 0% | 0 | 0% | 1 | 5% | | 51–80% | 0 | 0% | 0 | 0% | 51–80% | 2 | 14% | 1 | 17% | 3 | 16% | | 81–100% | 1 | 100% | 3 | 100% | 81–100% | 12 | 86% | 5 | 83% | 14 | 74% | | Stain intensity (tumor cells): | | | | | Stain intensity (tumor cells): | | | | | | | | None | 0 | 0% | 0 | 0% | None | 0 | 0% | 0 | 0% | 0 | 0% | | Weak | 1 | 100% | 3 | 100% | Weak | 8 | 57% | 4 | 67% | 11 | 58% | | Intermediate | 0 | 0% | 0 | 0% | Intermediate | 3 | 21% | 2 | 33% | 7 | 37% | | Strong | 0 | 0% | 0 | 0% | Strong | 3 | 21% | 0 | 0% | 1 | 5% |
| | | SEMA4F (Homo sapiens) | | SEMA4F (Mus musculus) | | | aRMS | eRMS | | aRMS | aRMS | UPS | | | # of samples | % of aRMS | # of samples | % of eRMS | | # of samples | % of aRMS | # of samples | % of eRMS | # of samples | % of UPS | | Stain present | 1 of 1 | 100% | 3 of 3 | 100% | Stain present | 12 of 16 | 75% | 6 of 7 | 86% | 18 of 33 | 55% | | % positive (tumor cells): | | | | | % positive (tumor cells): | | | | | | | | 0 | 0 | 0% | 0 | 0% | 0 | 0 | 0% | 0 | 0% | 0 | 0% | | 1–10% | 0 | 0% | 0 | 0% | 1–10% | 0 | 0% | 0 | 0% | 0 | 0% | | 11–50% | 0 | 0% | 0 | 0% | 11–50% | 0 | 0% | 0 | 0% | 1 | 6% | | 51–80% | 0 | 0% | 0 | 0% | 51–80% | 0 | 0% | 0 | 0% | 3 | 17% | | 81–100% | 1 | 100% | 3 | 100% | 81–100% | 12 | 100% | 6 | 100% | 14 | 78% | | Stain intensity (tumor cells): | | | | | Stain intensity (tumor cells): | | | | | | | | None | 0 | 0% | 0 | 0% | None | 0 | 0% | 0 | 0% | 0 | 0% | | Weak | 1 | 100% | 1 | 33% | Weak | 11 | 92% | 5 | 83% | 12 | 67% | | Intermediate | 0 | 0% | 1 | 33% | Intermediate | 1 | 8% | 1 | 17% | 5 | 28% | | Strong | 0 | 0% | 1 | 33% | Strong | 0 | 0% | 0 | 0% | 1 | 6% |
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