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Acceptance rate57%
Submission to final decision67 days
Acceptance to publication22 days
CiteScore1.280
Impact Factor1.242
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Atmospheric Corrosion Analysis and Rust Evolution Research of Q235 Carbon Steel at Different Exposure Stages in Chengdu Atmospheric Environment of China

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Scanning publishes international and interdisciplinary research focused on scanning electron, scanning probe, and scanning optical microscopies, and their advancement and applications.

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Research Article

SEM BSE 3D Image Analysis of Human Incus Bone Affected by Cholesteatoma Ascribes to Osteoclasts the Bone Erosion and VpSEM dEDX Analysis Reveals New Bone Formation

Bone erosion is considered a typical characteristic of advanced or complicated cholesteatoma (CHO), although it is still a matter of debate if bone erosion is due to osteoclast action, being the specific literature controversial. The purpose of this study was to apply a novel scanning characterization approach, the BSE 3D image analysis, to study the pathological erosion on the surface of human incus bone involved by CHO, in order to definitely assess the eventual osteoclastic resorptive action. To do this, a comparison of BSE 3D image of resorption lacunae (resorption pits) from osteoporotic human femur neck (indubitably of osteoclastic origin) with that of the incus was performed. Surface parameters (area, mean depth, and volume) were calculated by the software Hitachi MountainsMap© from BSE 3D-reconstructed images; results were then statistically analyzed by SPSS statistical software. Our findings showed that no significant differences exist between the two groups. This quantitative approach implements the morphological characterization, allowing us to state that surface erosion of the incus is due to osteoclast action. Moreover, our observation and processing image workflow are the first in the literature showing the presence not only of bone erosion but also of matrix vesicles releasing their content on collagen bundles and self-immuring osteocytes, all markers of new bone formation on incus bone surface. On the basis of recent literature, it has been hypothesized that inflammatory environment induced by CHO may trigger the osteoclast activity, eliciting bone erosion. The observed new bone formation probably takes place at a slower rate in respect to the normal bone turnover, and the process is uncoupled (as recently demonstrated for several inflammatory diseases that promote bone loss) thus resulting in an overall bone loss. Novel scanning characterization approaches used in this study allowed for the first time the 3D imaging of incus bone erosion and its quantitative measurement, opening a new era of quantitative SEM morphology.

Research Article

Multivariate Statistical Analysis on a SEM/EDS Phase Map of Rare Earth Minerals

The scanning electron microscope/X-ray energy dispersive spectrometer (SEM/EDS) system is widely applied to rare earth minerals (REMs) to qualitatively describe their mineralogy and quantitatively determine their composition. The performance of multivariate statistical analysis on the EDS raw dataset can enhance the efficiency and the accuracy of phase identification. In this work, the principal component analysis (PCA) and the blind source separation (BSS) algorithms were performed on an EDS map of a REM sample, assisting to achieve an efficient phase map analysis. The PCA significantly denoised the phase map and was used as a preprocessing step for the following BSS. The BSS separated the mixed EDS signals into a set of physically interpretable components, bringing convenience to the phase separation and identification. Through the comparison between the independent component analysis (ICA) and the nonnegative matrix factorization (NMF) algorithms, the NMF was confirmed to be more suitable for the EDS mapping analysis.

Research Article

Evaluation of Crack Formation and Propagation with Ultrasonic Root-End Preparation and Obturation Using a Digital Microscope and Optical Coherence Tomography

Objective. This study is aimed at determining (1) the effect of root-end resection, ultrasonic root-end preparation, and root-end filling on the incidence of crack formation and propagation by using a digital microscope (DM) and optical coherence tomography (OCT) and (2) the performance of OCT on the detection of cracks by comparing with microcomputed tomography (micro-CT) as a reference standard. Methodology. Thirty extracted lower incisors were endodontically treated and subjected to root-end resection and ultrasonic root-end cavity preparation. Then, the teeth were divided into three groups (, each), and the root-end cavity was either left unfilled or filled with mineral trioxide aggregate (MTA) or super-EBA. The resected surface was observed with OCT and DM after the root-end resection, ultrasonic root-end preparation, and root-end filling, and the frequency of incomplete and complete cracks were recorded. The observation was repeated after two weeks, one month, and two months, and micro-CT scans after two months were taken as the gold standard. Results. The DM results show dentinal crack formation in 47% of the samples following root-end resection and in 87% following ultrasonic preparation. After the ultrasonic preparation, no existing crack propagated to a complete crack, but new cracks were formed. MTA and super-EBA had no effect on crack formation. The Spearman correlation coefficient between OCT and DM was 0.186 (very weak correlation; ). Sensitivity and specificity in comparison to micro-CT were 0.50 and 0.55 in OCT and 1.00 and 0.35 in DM, respectively. McNemar’s test showed a significant difference between OCT and DM (). Conclusion. Apical resection and ultrasonic preparation could form dentinal cracks. OCT and DM showed different detection frequencies of cracks with very weak correlation. DM showed superior sensitivity compared with OCT.

Research Article

Thermodynamic Analysis of Myelin Basic Protein Adsorbed on Liquid Crystalline Dioleoylphosphatidylcholine Monolayer

To investigate the stability and dynamic characteristics of monolayer adsorbed on unsaturated lipid dioleoylphosphatidylcholine (DOPC) with varying concentrations of myelin basic protein (MBP), the system is studied by applying Langmuir technique and making atomic force microscope (AFM) observation, which is based on the mass conservation equation analysis method referred to in the thermodynamics theory. As indicated by surface pressure-mean molecular area () and surface pressure-adsorption time () isotherms, the physical properties of monolayer derived from the interaction of varying concentrations of MBP with liquid crystalline unsaturated lipid DOPC molecules were qualitatively studied. As revealed by surface morphology analysis with AFM, the micro region was expanded as the concentration of MBP in the subphase was on the increase, suggesting that hydrophobic interactions led to the MBP insertion, thus causing accumulation of the MBP on the surface of the monolayer. Experimental results have demonstrated that the partition coefficient of the interaction between MBP and unsaturated phospholipid DOPC and the molecular area of MBP adsorbed on the monolayer film was calculated using the mass conservation equation. In addition, not only does the varying concentration of MBP in the subphase exerts significant effects on the arrangement and conformation of DOPC monolayer, it also has certain guiding significance to exploring the structural changes to biofilm supramolecular aggregates as well as the pathogenesis and treatment of related diseases.

Research Article

Trueness and Precision of Two Intraoral Scanners: A Comparative In Vitro Study

The aim of this study was to evaluate the accuracy of two intraoral scanners used in the dental office. A molar fixed in a typodont was prepared for a ceramic onlay. The preparation was scanned using a high-resolution scanner (reference scanner) and saved as stereolithography (STL) format. The prepared resin molar was scanned again using the intraoral scanners, and all the scans were saved as well in STL format. All STL files were compared using metrology software (Geomagic Control X). Overlapping the meshes allowed the assessment of the scans in terms of trueness and precision. Based on the results of this study, the differences of trueness and precision between the intraoral scanners were minimal.

Research Article

Influence of AFM Tip Temperature on THF Hydrate Stability: Theoretical Model and Numerical Simulation

Atomic force microscopy (AFM) indentation is widely used to determine mechanical parameters of various materials. However, AFM tip may lead to phase transition of the cold sample in the region of contact area. It is a long-standing challenge that low-temperature phase-change materials (e.g., ice and hydrate) are hardly characterized by AFM, especially for clathrate hydrates. Here, with theoretical analysis and numerical simulation, we investigated the temperature influence of AFM tip on the tetrahydrofuran (THF) hydrate stability. At first, a steady-state model of heat conduction was established between a v-shaped probe and THF hydrate sample. The temperature of the tip was estimated at different laser spot positions and laser intensities. Through numerical simulation, the heat loss by air convection is less than 1% of the total laser heat, and the influence of ambient air on the AFM probe temperature can be neglected. Meanwhile, the local temperature in the region of contact area was also calculated at the THF hydrate temperature of 0°C, -10°C, -20°C, and -30°C. We found out that the AFM tip causes the cold THF hydrate to melt. The thermal melting thickness decreases with the reduction of laser intensity and THF hydrate temperature. On the contrary, it is positively correlated with the thickness of liquid-like layer on THF hydrate surface and is also linearly increased with the contact radius. This indicates that the thermal melting continues as the press-in depth of the tip into THF hydrate increases. The local temperature rises when the tip touches the THF hydrate. It is easier for THF hydrate to be melted by an external pressure. In addition, the proposed model may be useful for guiding force tests on low-temperature phase-change materials by the AFM indentation.

Scanning
 Journal metrics
Acceptance rate57%
Submission to final decision67 days
Acceptance to publication22 days
CiteScore1.280
Impact Factor1.242
 Submit
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