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Volume 2017 (2017), Article ID 1542156, 9 pages
https://doi.org/10.1155/2017/1542156
Research Article

Effects of Concentration and Surface Pressure on MBP Interaction with Cholesterol in Langmuir Films

School of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China

Correspondence should be addressed to Changchun Hao; nc.ude.unns@nuhcgnahcoah and Runguang Sun; nc.ude.unns@gnaugnurnus

Received 9 July 2017; Revised 31 August 2017; Accepted 12 September 2017; Published 7 November 2017

Academic Editor: Guosong Wu

Copyright © 2017 Lei Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Predicting the mechanism of MBP binding to cholesterol is meaningful in understanding how MBP participate in lateral membrane organization. The interaction of MBP with cholesterol monolayer was investigated at three surface pressures on 10 mM Tris-HCl buffer with the different concentrations of MBP. The results show that π-A isotherms shift to larger molecular area at all pressures. By means of analyzing π-T curves, a surface pressure increase was obtained. Results indicated that the greater the protein concentration in the subphase, the larger the increase of surface pressure. In addition, changes in monolayer surface morphology and domain formation were performed by AFM. These results provide more direct and convincing evidence for the MBP interaction with cholesterol. The MBP-cholesterol interaction suggests a significant concentrations and surface pressure dependence and is probably governed by hydrogen bonds. The date presented could help to understand at least one of the molecular mechanisms through which MBP affects lateral organization of the cholesterol membrane.