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Volume 2017, Article ID 9650420, 7 pages
Research Article

Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3

1Department of Restorative Dentistry, Federal University of Parana (UFPR), Curitiba, PR, Brazil
2School of Dentistry, Tuiuti University of Parana (UTP), Curitiba, PR, Brazil
3Department of Materials Engineering, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil
4Department of Biomedicine, Tuiuti University of Parana (UTP), Curitiba, PR, Brazil
5Department of Pharmacy, Federal University of Parana (UFPR), Curitiba, PR, Brazil

Correspondence should be addressed to Yasmine Mendes Pupo; moc.liamtoh@sednemenimsay

Received 13 July 2016; Accepted 17 October 2016; Published 10 January 2017

Academic Editor: Jessem Landoulsi

Copyright © 2017 Yasmine Mendes Pupo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to evaluate in fibroblast cultures the direct cytotoxic effects of etch-and-rinse, self-etch, and universal adhesive systems. The sterile glass cover slips () were then immersed in culture medium to obtain the eluates for the experimental groups: (1) Adper™ Single Bond 2; (2) Ambar; (3) Adper™ Scotchbond™ Multi-Purpose; (4) Scotchbond™ Universal; (5) Ambar Universal; and (6) OptiBond All-In-One. As a negative control, sterile glass cover slips were immersed in culture medium only. After 24 h, the eluate obtained was applied on fibroblast culture. Cell viability and cell morphology were evaluated by MTT assay and SEM, respectively. Data were analyzed by Kruskal–Wallis and Mann–Whitney tests (). All adhesive systems except universal reduced cell viability in 3T3 cells to between 26.04% and 56.57%, and Scotchbond Universal and Ambar Universal reduced cell viability to 2.13% and 3.57%, respectively, when compared to the negative control. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, improvements in universal adhesive system formulations and their mechanisms of action are not accompanied by increased toxicity compared with those in other systems, warranting commitment to the use of these dentin-pulp complexes.