Resolution-Free Accurate DNA Contour Length Estimation from Atomic Force Microscopy Images

Algorithm 1 : ID number derived from shape number.

Require: shapes, j = 1 ∼ 12 and their respective SESN or ESSN s_kj = s₁s₂s₃

Ensure: Unique ID numbers for all k₁ ∼ k₁₂ shapes.

1: forj = 1 ∼ 12 do

2:  take the k_j’s SN: s = {s₁, s₂, s₃}

3:  ifs₁ ≥ 4 then

4:   take 8’s complement for this sequence of s, for bi-direction and mirroring re-ordering, but

5:   ifs₂ = 0 or s₃ = 0 then

6:    keep s₂ or s₃ zero

7:   else

8:    s₂ = 8 − s₂ and s₃ = 8 − s₃

9:  end if

10: end if

11: Take the new S (with s₁ ≤ 4), and further re-order s₂ and s₃, to match the rotational varieties, by

12: ifs₂ > s₃then

13:  switch s₂ with s₃

14: else

15:  retain original order

16: end if

17: Find final ID number for given SN s_kj

18: end for

Algorithm 1 : ID number derived from shape number.