A Glutamine-Rich Factor Affects Stem Cell Genesis in Leech

Figure 5

Transient knockdown of Tpr mRNA as monitored by semi-quantitative PCR. Antisense oligonucleotide TprB was microinjected into the D macromere of $~$50 embryos, and $~$12 embryos were harvested 6 (stage 6), 12 (stage 7), 24 (early stage 8) and 45 (mid-stage 8) hours later, respectively. Tpr mRNA levels were apparent by 24 hours post-injection in proliferated embryos. Control (no injection) and sense (TprC) microinjections were conducted in the same experimental clutch and harvested at 6 (stage 6) and 12 (stage 7) hours post-injection, respectively. Lanes were normalized with 18S rRNA primers.