Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units

Table 1

Details of transplanted cord units. Cellular characteristics and HlA types of the cord units used in the transplant are given. No attempt was made to have matching tissue types in the unmatched cords. The matched cord differed from the patient in blood group Patient A+ matched cord O+. Tissue type of matched cord was identical at Class 1 DR, and DP were fully matched but there was a DQ mismatch. All cord donations were female and CMV antibody negative Genotypes are the allele ID’s for the markers used at the CTLA4 and TNFA loci (refs). The matched cord cell dose was substantially less than the recommended dose for engrafting an adult ($\mathit{0.8}\times {\mathit{10}}^{\mathit{7}}$/kg) but engraftment of granulocytes was swift and uncomplicated. NK-not known.

Sample description	Blood group and Hla types of transfused cord units	Total NUC cell count $\times \mathrm{\hspace{0.17em}\hspace{0.17em}}{10}^7$	Nucleated cell count ${10}^7$/kg	MNC $\times \hspace{0.17em}{10}^7$ Total	CD34 $\times {10}^8$ Total	CTLA4 microsatellite genotype	TNF microsatellite genotype
Patient leukaemia	—	—	—	—	—	27, 28	a2,7; d3,4
Matched cord	O Pos and A29, A68, B44, B1402/04/06 Cw08, Cw1601, DRB1, DQB1, DPB1	102	1.46	56.2 (0.8 $\times {10}^7$/kg)	- NK	16, 19	a2,7; d4,4
Nonmatch cord 1	A Pos and A2, A11, B8, B35, Cw7, Cw4/18, DR1	66.6	0.95	- NK	0.36	8, 16	a6,10; d3,3
Nonmatch cord 2	A Pos and A1, A11, B18, B38, Cw12, Cw7, DR13, DR15	132.8	1.89	- NK	0.292	8, 8	a2,2; d1,4
Nonmatch cord 3	A Pos and A1, A26, B8, B49, Cw7, DR1, DR17	159.8	2.28	- NK	0.639	8, 22	a2,5; d1,3
Nonmatch cord 4	A Pos and A1, A2, B8, B27, Cw2, Cw7, DR17	80.2	1.15	- NK	0.217	8, 19	a2,2; d1,4
Nonmatch cord 5	A Pos and A2, B44, B60, Cw5, Cw10, DR13, DR15	64.6	0.92	- NK	0.058	19, 22	a4,11; d3,3
Nonmatch cord 6	A Pos and A2, A3, B7, B60, Cw7, Cw10, DR4, DR17	91.4	1.31	- NK	0.165	8, 8	a2,11; d3,4
Patient blood +21 days		—	—	—	—	16, 19	a2,7; d4,4