Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells
Figure 1
(a)–(c) Photomicrographs of NPI after culture for 1 (a), 6 (b), and 10 (c) days on T25 tissue flasks for adherent cell growth. (d) Light field (left) and fluorescence (right) photomicrographs of Ba-AG microcapsules containing SC. Fluorescence micrographs were obtained after staining with EB+FDA to assess SC viability. (e) Insulin secretory patterns of control NPI cell monolayers alone after 14 days (open bars) or NPI cell monolayers, cocultivated with microencapsulated SC for 7 (gray bars), 14 (hatched bars), and 21 days (filled bars) of culture. During static incubation, the cells were treated with the indicated concentrations of glucose. Data represent the average of 3 independent experiments; each insulin determination was performed in triplicate ±SD.
