Acceleration of Functional Maturation and Differentiation of Neonatal Porcine Islet Cell Monolayers Shortly In Vitro Cocultured with Microencapsulated Sertoli Cells

Table 2

Immunohistochemical analysis of differentiation markers of NPI monolayers cultured alone or in the presence of ESC.

Cells (days)	Ck7+/Insulin+ (%)	PDX-1+/Insulin+ (%)	Insulin+/c-kit+ (%)	PDX-1/c-kit+ (%)
C (7)	58.5 ± 1.4	14.8 ± 3.9	10.0 ± 1.7	13.1 ± 2.3
+ESC (7)	55.3 ± 5.5	14.3 ± 3.0	9.7 ± 4.0	14.3 ± 9.7
C (14)	50.0 ± 3.8	20.7 ± 3.1	14.0 ± 3.7	25.7 ± 10.0
+ESC (14)	40.2 ± 2.4	33.3 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{5.1}^{*}$	26.0 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{0.9}^{*}$	31.1 ± 13.5
C (21)	45.6 ± 4.7	29.5 ± 4.9	15.8 ± 2.9	45.1 ± 11.0
+ESC (21)	32.9 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{4.1}^{*}$	74.8 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{4.9}^{*}$	38.3 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{5.2}^{*}$	71.8 ± $\mathrm{\hspace{0.17em}\hspace{0.17em}}{10.8}^{*}$

C: Control untreated NPI monolayers.
+ESC: NPI monolayers cocultured in the presence of encapsulated Sertoli cells.
Data represent the percentage of positive cells and are given as means ± SD ($n$ = 3).
*P < .050.