Alteration of Differentiation Potentials by Modulating GATA Transcription Factors in Murine Embryonic Stem Cells

Table 1

Information on genes analyzed by real-time RT-PCR using Taqman assay.

Gene Symbol	NCBI Gene Reference	Target Exons	Context Sequence
Tbp	NM_013684,NM_013684	3	ATCCCAAGCGATTTGCTGCAGTCAT
Gata4	NM_008092,AF179424,U85046, M98339,AB075549	3	CGCCGCCTGTCCGCTTCCCGCCGGG
Gata5	NM_008093,U84725	1	AGGACCAGCTTCGTACCTGACTTCT
Gata6	NM_010258,AF179425	5	CTCAGGGGTAGGGGCATCAGTGATG
Dab2	NM_023118,U18869	1	TAGCTAGTCCGTGTACTTTGTGGGT
Pou5f1	NM_013633,X52437,M34381,BC068268	2	GCGTTCTCTTTGGAAAGGTGTTCAG
Afp	NM_007423,V00743,BC066206, AK075972, AK010934,AK076053,AK076197	3	GTGTTTAGAAAGCCAGCTATCTGTG
Mest	NM_008590,D16262,AK031718,AK032881,AK034949,BC006639	1	TCGCTTGCGCAGGATGAGAGAGTGG
Lpr2	AF197160,BC040788	11	CTATGCAGAGATGGACACTGAGCAA
Hnf4a	NM_008261,D29015,BC039220	8	ATGCTTCTCGGAGGGTCTGCCAGTG
Lrp1	NM_008512,X67469,AF367720	15	CCTGCTTGGCGAACCCATCCTACGT
Lama1	NM_008480,J04064	19	AAACTGCCGAGCCTGTGACTGCCAC
Col3a1	NM_009930,BC058724,M18933,AK029212, BC052398,AK013329,AK041115,AK048546,BC043089	4	GTGGCCAAAATTATTCTCCCCAATT
Tdgf1	NM_011562,M87321	15	AAGACTGGGGAAACAGAGTGGATTG
Tpm1	NM_024427,X64831,M22479,BC026720, AK002271,AK003175,AK032942,AK077713	1	CGGAGCAAGCAGCTGGAAGATGAGC
H19	X58196,BC025150	1	GGACTGGAGACTAGGCCAGGTCTCC

Tbp was used as normalization control.
Afp: alpha fetal protein; Col3a1: collagen III alpha 1; H19: fetal liver RNA transcript; Lama 1: laminin alpha 1 gene; Lrp: lipoprotein-related receptor protein; Mest: mesoderm-specific transcript; Pou5f1: Oct-3/4; Tdgf1: Teratocarcinoma derived growth factor 1 (cripto); Tbp: TATA box binding protein; Tpm1: tropomyosin 1, alpha.