Stem Cells International

Research Article

Alteration of Differentiation Potentials by Modulating GATA Transcription Factors in Murine Embryonic Stem Cells

Table 2

Limited list of genes whose expression is significantly altered following retinoic acid treatment of ES cells grow in monolayer cultures.


	WT	GATA4 (−/−)	GATA5 (−/−)	GATA6 (−/−)

Dab2	4.38, 3.77	2.00, 1.80	4.09, 3.24	0.05, 0.48
GATA4	3.32	0.22	2.58	0.91
GATA6	2.04, 2.74, 2.51, 2.38	2.15, 2.08	1.94	0.41, 0.01
collagen IVa1	2.58, 2.30	2.86, 2.73	2.94, 2.72	2.72, 3.00, 2.84
collagen IVa2	1.88	2.66	2.48	2.48, 3.03
laminin alpha1	2.52	2.59	3.79	0.20
laminin beta1	2.74, 2.65	2.13	3.05	2.01
laminin gamma1	2.27, 2.00	2.20	2.38, 2.94, 2.02	2.90
keratin gene 8	3.20, 3.005, 2.301	2.59, 2.2, 2.17	2.27, 1.61	3.08, 3.05, 2.68, 1.99
procollagen III	0.87	0.48	1.07	3.96
lrp1	2.55	1.76	2.90, 2.35	0.15, 0.27
lrp2	2.28, 2.15	1.83	2.37	0.09, 0.65
Talin	2.52	2.87	2.93	3.62
fetal liver H19	2.20, 2.65, 2.35	2.65, 2.38	3.86, 3.80, 3.68	3.82, 3.80
igf2	2.29	3.26	2.66	3.75
mesoderm transcript	1.29	1.77	2.09	3.12
tropomyosin 1alpha	1.36, 1.37, 1.32	2.2, 2.17, 2.1, 1.9	1.42, 1.38, 1.35	3.68, 3.64, 2.94
alpha fetoprotein	3.0	3.09	3.61	3.22
POU domain class 5	0.66	0.00	−2.01	−5.18
Arc	−2.37	−1.10	−0.79	−1.39
Ran small G protein	−2.28	−0.23	−0.40	−1.65

The monolayer cultures of ES cells were treated with or without retinoic acid (RA) for 4 days and mRNA was isolated for expression array analysis. A selected list of genes of interest with large-fold expression changes following RA treatment is presented. The fold-changes in expression between with or without RA can be calculated as 2n. The number “n” is listed in the table. Multiple numbers in a category indicate several cDNA entries of the same gene on the cDNA chip. The presence of hybridization signal for a GATA transcript in cells that were homozygous knockout of the GATA gene is likely due to the presence of the mutant GATA transcripts that are inactive. The authors will provide the full data of the expression array experiments if requested. The complete list of differentially expressed genes will be found online: http://www.ncbi.nlm.nih.gov/projects/geo/, Accession “GPL4486”.