Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Endotoxin-Induced Oxidation of Plasma Cysteine and Glutathione in Mice
Effect of BMDMSC infusion on endotoxin-induced depletion of plasma GSH and oxidation of GSH/GSSG redox state. C57BL/6J mice were treated with 1mg/kg i.p LPS followed by i.v infusion of either 500,000 BMDMSC (LPS/BMDMSC), Lung fibroblasts (LPS/Lung fibroblast), or 0.1 mL Saline (LPS/Saline) alone. At 2 hours, 6 hours, and 24 hours mice were sacrificed and plasma was collected for HPLC analysis of GSH (a) and GSSG (b). GSH/GSSG (c) was calculated from the GSH and GSSG concentrations using the Nernst equation. Data are expressed as means + SEM. aValues in LPS/BMDMSC group significantly different from time-matched LPS/Saline group. bValues in LPS/BMDMSC group significantly different from time-matched LPS/Lung fibroblast group.