Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
Figure 1
(a) The explant from the normal bone marrow showed healthy cell generation at day 3 of culture. (b) Explant from the aplastic bone marrow showed stunted cellular growth with little or no cellular generation at day 3 of culture. (c) At day 7 of culture, explant from the normal bone marrow demonstrated a cumulative cell production with the efficacy of stromal matrix formation which was completely absent in the explant from aplastic marrow (d). (e) After 11 days of culture, the cells released from the normal bone marrow created various stromal microenvironments like structures throughout the culture dish distal from the explant compared to the aplastic bone marrow where no such stromal matrix generation was observed with predominant appearance of immature stromal precursors and stromal fibroblasts (f). (g) After day 16 of culture, the cells from the normal bone marrow produced a coordinated network of mature stromal matrix of fibroblastic cells upon which small hematopoietic cellular colony formation can be noticed. (h) After 16 days of the culture, the cells from the aplastic bone marrow failed to generate a functional stromal matrix that can sustain hematopoiesis with abundance of immature giant stromal precursors and fibroblastic cells. (i) After 19 days of culture, the normal marrow generated stromal matrix was appeared to be even more organized with appearance of large hematopoietic colonies associated with it, compared to the aplastic marrow where no such functional stromal matrix formation with associated cellular colony generation was observed (j).
