Table 1: Summary of culture supplements used for expansion.

SupplementsAdvantagesDisadvantages

Flt3/Flk2 ligandPopularly used in numerous studies; serum-free conditions availableExpensive for large-scale culture; difficult to determine the molecular mechanisms of the action; too many combinations, which are not standardized; some ILs may not be necessary
Stem cell factor
ILs
Thrombopoietin
Feeder cell No need to supply expensive growth factors Complicated culture systems
Notch ligandEffective; signaling pathway is relatively clearImmobilization required; could induce apoptosis under certain conditions
Aryl hydrocarbon receptor antagonistsDefined chemicals, can reduce the costVery new, further studies needed