Human Fetal Liver: An In Vitro Model of Erythropoiesis
Figure 3
Maturation of FL-derived erythroid cells. (a) FACS analyses of transferrin receptor (CD71), CD36 and glycophorin A (CD235a) expression on days 8 and 18 of liquid culture. Data are from one representative experiment and the green, light blue and dark blue, tracings represent the negative control and cells from days 8 and 18 of culture, respectively. (b) Photographs of the cells on days 8, 10, 15, and 18 of liquid culture after May-Grünwald-Giemsa staining. Every stage of erythroid maturation is represented: proerythroblast, basophilic erythroblast, polychromatophilic erythroblast, orthochromatic erythroblast, and RBCs (magnification ×50).
