The Role of Glucose, Serum, and Three-Dimensional Cell Culture on the Metabolism of Bone Marrow-Derived Mesenchymal Stem Cells
Figure 4
Fluorescence microscopy images indicating the viability of BMSCs in three-dimensional culture after the second and sixth days (increasing glucose concentrations from left to right; increasing collagen densities from top to bottom). Calcein-AM indicates living cells by emitting green light, while ethidium bromide indicates dead cells by emitting red light. After day 2, the 0.5 mM environment resulted in low viability, while the 25.0 mM environment resulted in high viability. By day 6, the 0.5 mM environment and the 25.0 mM−2.5 mg/mL group resulted in low viability, while the 25.0 mM−5.0 mg/mL group resulted in high viability and confluency. (40x objective; scale bar represents 100 μm).
