Research Article

The Role of Glucose, Serum, and Three-Dimensional Cell Culture on the Metabolism of Bone Marrow-Derived Mesenchymal Stem Cells

Table 1

Summary of two-dimensional L/P ratios and L/G ratios. L/P ratios indicate that groups with a higher starting glucose concentration exhibited a more anaerobic characteristic. This trend was similar in the L/G ratios for the 2% serum group, but not for either the 5% or 10% serum groups. (a: significantly different from corresponding value in the 5.0 mg/mL group; b: significantly different from the 25.0 mM value within the same collagen group; f: significantly different from 5.0 mM value within same serum group; 𝑃 = . 0 5 ).
(a) Two-dimensional lactate-phosphate (L/P) ratios.

Glucose conc. 2% serum5% serum10% serum
MeanSt. Dev.N MeanSt. Dev.N MeanSt. Dev.N

Day 40.5 mM2.412.3632.852.9265.923.483
1.0 mM0.59b0.9394.77b4.18105.613.027
5.0 mM8.798.521223.4617.011052.4834.088
25.0 mM14.9813.121236.0534.251250.0132.6211

Day 60.5 mM0.170.3489.5717.8944.154.834
1.0 mM10.0625.281126.5811.34631.7637.434
5.0 mM51.3717.226134.35111.218234.68549.510
25.0 mM71.4959.7110113.1876.512114.8152.369

(b) Two-dimensional lactate-glucose (L/G) ratios.

Glucose conc. 2% serum5% serum10% serum
MeanSt. Dev.N MeanSt. Dev.N MeanSt. Dev.N

Day 40.5 mM0.72a0.4581.470.0960.570.6212
1.0 mM0.520.55121.81b1.44122.713.6510
5.0 mM1.190.89122.21.42122.091.4412
25.0 mM1.531.56121.91.49122.852.6712

Day 60.5 mM0.65f0.4991.942.5280.590.6312
1.0 mM0.47f0.49123.652.61213.5322.579
5.0 mM2.391.75122.992.29122.692.0712
25.0 mM1.561.2121.951.45122.321.9112