Detection of Intranasally Delivered Bone Marrow-Derived Mesenchymal Stromal Cells in the Lesioned Mouse Brain: A Cautionary Report

Figure 6

Detection of MSC-EGFPs after intrastriatal delivery. (a) 1d after implantation, endogenous eGFP fluorescence from MSCs is found proximal to the injector needle track. Tissue counterstained with DAPI. (b) At higher magnification (40x), fibroblast-like structure of implanted MSC-EGFPs can be seen. Fluorescence is detected on GFP (c), but not Texas Red (c′) emission filters. In the OB, autofluorescence is detected on both the GFP (d) and Texas Red (d′) emission filters. (e) 7d after implantation, endogenous eGFP fluorescence from MSCs is found around the injector needle track. Tissue counterstained with DAPI. (f) At higher magnification (40x), implanted MSC-EGFPs can be seen. Fluorescence is detected on GFP (g), and Texas Red (g′) emission filters, although there is no overlap with GFP signal. In the OB, autofluorescence is detected on both the GFP (h) and Texas Red (h′) emission filters. Abbreviations: cc: corpus callosum; lv: lateral ventricle.