Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R mice
Figure 1
MNC from AB and CB generate great numbers of EBs under HEMA conditions. (a) Cell number (as Fold Increase, FI, with respect to day 0), (b) maturation profile (cytofluorimetric analysis on the basis of the expression of CD36 and CD235a), and (c) representative morphology (by May-Grunwald staining) of EBs generated in HEMA culture seeded with MNC from either CB or AB. The ability of CB and AB EBs obtained at day 10 to proceed along the maturation pathway after 4 days of culture in the presence of EPO only is also compared (b). The flow cytometric profile used to define EBs maturation is presented in Supplemental Figure 3. The flow charts are representative of those obtained in at least three independent experiments with MNC from different CB or AB donors. The numbers in the quadrants present the frequency of EBs in the gates R1 to R4. Frequencies obtained in multiple experiments are presented as mean (±SD) in Table 1.
