Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R mice
Figure 2
Adhesion receptor profiling during in vitro maturation of EBs expanded from AB (a) and CB (b). Three color flow cytometry analysis for CD36 and CD235a in combination with either CXCR4 (CD184), PSGL-1 (CD162), or VLA-4 (CD49d) of EBs obtained at day 10 of HEMA culture from AB and CB MNC (Prol 10) and at day 1 (Diff Day 1) and 4 (Diff Day 4) of maturation with EPO alone, as indicated. EBs were divided into 4 maturation classes defined by the levels of CD36 and CD235a expression identified by the gates R1 to R4 and corresponding to Class 1 to 4 (see Supplemental Figure 3 for further details).
