Recovery and Biodistribution of Ex Vivo Expanded Human Erythroblasts Injected into NOD/SCID/IL2R𝜸^null mice

Table 2

Frequency and total number of cells expressing the human CD235a in bone marrow and spleen of NOD/SCID/IL2Rγ ^null mice transfused 4 days earlier with CFSE-labeled EBs. Both CFSE-positive and CFSE-negative human CD235a^pos cells were detected in the organs of the animals after transfusion, an indication that some of the human Ebs had proliferated in vivo.

	CSFE^+CD235a^+ (%)	CSFE^-CD235a^+ (%)	CSFE^++CSFE^−CD235a^+−control (%)	Total no. of CD235^+ cells/organ*
BM
Control	0.3	0.34	0
Transfused mice (2 mice)	0.53–0.75	1.1–1.07	0.99–1.18	3.0-3.5×10^6
Spleen
Control	1.02	1.04	0
Transfused mice (2 mice)	3.34–5.75	1.3–1.6	2.57–5.29	2.6-5.3×10^6

The frequency of human CD235a⁺ cells was obtained after subtracting the background signal observed in untreated controls.
*The total number of cells per organ was calculated assuming that a mouse contain 300×10⁶ BM and 100×10⁶ spleen cells [19].