Research Article

Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro

Figure 2

Generation & characterization of toxic milk iPS cells. (a) iPSCs generated from fibroblasts isolated from toxic-milk mice using retroviral vectors expressing human OCT4, KLF4, SOX2 and c-MYC. (b) iPSCs were subcloned to obtain better colony morphology. (c)–(f) Subcloned iPSCs stained positive for murine Oct4 and Nanog, whilst DAPI was used to stain the nuclei. (g) Staining for alkaline phosphatase expression of toxic-milk iPSC subclones. (h) Karyotype analysis of DAPI stained metaphase spreads. (i) qRT PCR analysis for endogenous Oct4, Nanog and Sox2 expression in toxic-milk iPSCs and OG2 ESCs compared to mouse embryonic stem cells. (j)–(l) Teratoma sections depicting formation of neuroectoderm, mesoderm (connective tissue), and endoderm.
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