Spleen as a Site for Hematopoiesis of a Distinct Antigen Presenting Cell Type

<table>Isolation of L-DC (LIVE-DC) in spleen amongst other DC subsets. Spleens were depleted of T and B cells using antibody-coated magnetic beads and then stained with antibodies specific for CD11c, CD11b, CD8<i>α</i> and MHC-II ahead of flow cytometric analysis. DC subsets were identified based on multiple parameters of forward scatter (FSC), side scatter (SSC), marker expression and endocytosis after intravenous administration of FITC-OVA (0.6 mg/mouse) 24 hours previously.</table>

Stem Cells International

fig3

Figure 3

Figure 3: Spleen as a Site for Hematopoiesis of a Distinct Antigen Presenting Cell Type 