Spleen as a Site for Hematopoiesis of a Distinct Antigen Presenting Cell Type

Figure 4

Relatedness of transcriptome between L-DC (LIVE-DC) and other spleen myeloid subsets. Myeloid and DC subsets were isolated from spleen by antibody staining and cell sorting using flow cytometry as demonstrated in Figure 3. RNA was prepared from DC subsets described in Figure 2, and from granulocytes (GRAN) as Gr-1⁺CD11b⁺CD11c^- cells and monocytes (MONO) as CD11b⁺CD11c⁻MHC-II⁻ cells. RNA was used to probe Affymetrix Gene 1.0ST microarrays. Data show Principal Components Analysis (1&2) reflecting degree of relatedness in overall gene expression between subsets.