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Stem Cells International
Volume 2012 (2012), Article ID 108340, 10 pages
http://dx.doi.org/10.1155/2012/108340
Research Article

Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions

1Department of Ophthalmology, Ophthalmology Research Laboratories, The Gavin Herbert Eye Institute, University of California, Irvine, CA 92697, USA
2Department of Ophthalmology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China

Received 30 September 2011; Accepted 3 February 2012

Academic Editor: Heuy-Ching Hetty Wang

Copyright © 2012 Jing Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Neural progenitor cells (NPCs) of feline origin (cNPCs) have demonstrated utility in transplantation experiments, yet are difficult to grow in culture beyond the 1 month time frame. Here we use an enriched, serum-free base medium (Ultraculture) and report the successful long-term propagation of these cells. Primary cultures were derived from fetal brain tissue and passaged in DMEM/F12-based or Ultraculture-based proliferation media, both in the presence of EGF + bFGF. Cells in standard DMEM/F12-based medium ceased to proliferate by 1-month, whereas the cells in the Ultraculture-based medium continued to grow for at least 5 months (end of study) with no evidence of senescence. The Ultraculture-based cultures expressed lower levels of progenitor and lineage-associated markers under proliferation conditions but retained multipotency as evidenced by the ability to differentiate into neurons and glia following growth factor removal in the presence of FBS. Importantly, later passage cNPCs did not develop chromosomal aberrations.