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Stem Cells International
Volume 2012, Article ID 303610, 7 pages
http://dx.doi.org/10.1155/2012/303610
Clinical Study

Optimization of Parameters for a More Efficient Use of Adipose-Derived Stem Cells in Regenerative Medicine Therapies

1Institute of Biosciences, University of São Paulo, 05508-090 São Paulo, Brazil
2Department of Plastic Surgery, Faculty of Medicine, University of São Paulo, 01246903 São Paulo, Brazil

Received 27 September 2011; Revised 15 December 2011; Accepted 3 January 2012

Academic Editor: Peter Schlenke

Copyright © 2012 Meire Aguena et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation is dependent on the number of ASCs. In a sample of 10 donors, we found a higher concentration of nucleated cells in the lower abdomen compared to flank ( ). In a sample of 6 donors we did not find differences in the cell yield obtained by manual or pump-assisted aspiration ( ). We suggest that the increase in the number of ASCs in the reinjected fat may enhance the efficiency of newly formed adipose tissue and that the anatomical region from which to harvest fat tissue needs to be considered to optimize the number of ASCs in the harvested tissue. Finally, pump-assisted aspiration can be used without any significant harm to the viability of cells.