Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study
Figure 6
Myogenic differentiation. Phase contrast microscopy of cultures grown in myogenic medium for 21 days (a) at low magnification, immunofluorescence for myogenin in cells cultured in myogenic medium for 21 days; (b) myogenin stained with primary antibody against and with a secondary antibody anti-mouse (conjugated with Alexa Fluor 488); (c) merged with DAPI (blue, nuclei); (d) negative control with DAPI and secondary antibody only.