Secreted Factors from Bone Marrow Stromal Cells Upregulate IL-10 and Reverse Acute Kidney Injury

Figure 4

BMSC-CM prevents apoptosis in the outer medulla. (a) TUNEL staining was performed on sections of formalin fixed and paraffin-embedded kidneys from rats administered a lethal dose of cisplatin (7.5 mg/kg) and sacrificed at 5 days following cisplatin administration. The arrows indicate TUNEL positive cells. The rats were treated at 3, 9, 24, 48 and 72 hours following cisplatin administration, with 1 mL of either physiological saline (𝑛=4), or BMSC conditioned medium (𝑛=3) i.v. (b) Quantification of the TUNEL positive cells per field of view. Scale bar = 200 μm. ^∗𝑃<0.01 by Student’s T-test.

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