Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A
Figure 3
Human myoblasts immunosuppression is mediated by Gal-1 and Sema-3A. (a, b, c) RT-PCR, flow cytometric, and immunoprecipitation analysis of Gal-1 and Sema-3A expression and secretion by myoblasts. MSCs were used as control. Data are means ± SEM of triplicates from one representative of at least 10 independent experiments. P values from Student’s t-test are indicated (**P < 0.01, ***P < 0.001). (d) Neutralization of Gal-1 and Sema-3A restores PBMCs proliferation. PBMCs from eight independent donors were cultured alone (A+B) or with myoblasts (ratio 1/5) in the presence of increasing concentration of anti-Gal-1 or anti-Sema-3A antibodies (0 to 40 μg/mL). Data are means ± SD of triplicates from one representative of at least 10 independent experiments. P values from Student’s t-test are indicated (*P <0.05, **P < 0.01, and ***P < 0.001).
