Research Article

Directed Fusion of Mesenchymal Stem Cells with Cardiomyocytes via VSV-G Facilitates Stem Cell Programming

Figure 2

VSV-G facilitates vMSC-CM fusion. Four hours after pH-induced fusion, samples were harvested and analyzed using fluorescence deconvolution microscopy and flow cytometry for events displaying both CellTracker fluorescent probes. (a) Representative vMSC-CM fusion event (white arrow). CMs were labeled with CellTracker green and vMSCs were labeled with CellTracker red. Scale bar = 50 μm. (b) Effect of VSV-G on vMSC-CM fusion at pH = 5.5. Fusion events were increased 3.5-fold with VSV-G at low pH. (c) Effect of pH on VSV-G-mediated MSC-CM fusion. vMSC-CM fusion was inhibited by pH = 6.5 and 7.5 (inactive form of VSV-G) .
414038.fig.002a
(a)
414038.fig.002b
(b)
414038.fig.002c
(c)