Research Article

Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC

Figure 2

Generation of EiPS cells. (a) Morphology of EiPS single colony growing from individual fibroblast. (b) Typical colony of EiPS cells on the MEF and colony selection. (c) Cutting individual colony for manual passaging. (d) Passaged pieces on the MEF. (e) Alkaline phosphatase activity of EiPS cells scale bar 200 μM. (f) Chromosome spread of EiPS cells.
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