Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
Figure 5
Characterization of cardiac tissue after SEC. Representative photomicrographs of tissue sections of formalin-fixed paraffin-embedded atrial tissue. The atrial appendage from a patient with end-stage heart failure was removed at the time of heart transplantation and cultured for 28 days using the SEC method. (a) Day 28 tissue explant stained with hematoxylin (blue) and eosin (red). Note preserved cardiomyocyte morphology and small nucleated interstitial cells (arrows). Magnification 630. (b) Day 28 tissue explant stained with Trichrome. Note, a modest increase in interstitial connective tissue (blue; arrows). Magnification 630. (c) Day 28 tissue explant immunostained to demonstrate proliferating interstitial nucleated cells. Proliferating cell nuclear antigen/PCNApositive cells are brown (DAB) with hematoxylin counterstain (blue). Magnification 400. (d) Day 28 tissue explant immunostained for muscle-specific actin are brown (DAB) and hematoxylin counterstain (blue). Note conservation of the myocellular contractile elements with visible cross-striations (arrow). Magnification 630.
