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Stem Cells International
Volume 2012 (2012), Article ID 485950, 15 pages
http://dx.doi.org/10.1155/2012/485950
Research Article

Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity

1Wake Forest School of Medicine, Institute for Regenerative Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA
2Wake Forest University, Medical Center Blvd., Winston-Salem, NC 27157, USA
3Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA

Received 3 March 2012; Revised 9 May 2012; Accepted 29 May 2012

Academic Editor: Toshio Nikaido

Copyright © 2012 Patricia G. Wilson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Atala, “Engineering tissues, organs and cells,” Journal of Tissue Engineering and Regenerative Medicine, vol. 1, no. 2, pp. 83–96, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. H. Reinshagen, C. Auw-Haedrich, R. V. Sorg et al., “Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits,” Acta Ophthalmologica, vol. 89, no. 8, pp. 741–748, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. F. Mosna, L. Sensebé, and M. Krampera, “Human bone marrow and adipose tissue mesenchymal stem cells: a user's guide,” Stem Cells and Development, vol. 19, no. 10, pp. 1449–1470, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. I. Antonucci, A. Pantalone, S. Tetè et al., “Amniotic fluid stem cells: a promising therapeutic resource for cell-based regenerative therapy,” Current Pharmaceutical Design, vol. 18, no. 13, pp. 1846–1863, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. A. Hilfiker, C. Kasper, R. Hass, and A. Haverich, “Mesenchymal stem cells and progenitor cells in connective tissue engineering and regenerative medicine: is there a future for transplantation?” Langenbeck's Archives of Surgery, vol. 396, no. 4, pp. 489–497, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Joo, I. K. Ko, A. Atala, J. J. Yoo, and S. J. Lee, “Amniotic fluid-derived stem cells in regenerative medicine research,” Archives of Pharmacal Research, vol. 35, no. 2, pp. 271–280, 2012. View at Publisher · View at Google Scholar · View at Scopus
  7. R. Soler, C. Fllhase, A. Hanson, L. Campeau, C. Santos, and K.-E. Andersson, “Stem cell therapy ameliorates bladder dysfunction in an animal model of parkinson disease,” Journal of Urology, vol. 187, no. 4, pp. 1491–1497, 2012. View at Publisher · View at Google Scholar · View at Scopus
  8. S. A. I. Kolli, S. Ahmad, M. Lako, and F. Figueiredo, “Successful clinical implementation of corneal epithelial stem cell therapy for treatment of unilateral limbal stem cell deficiency,” Stem Cells, vol. 28, no. 3, pp. 597–610, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. P. M. Chen, M. L. Yen, K. J. Liu, H. K. Sytwu, and B. L. Yen, “Immunomodulatory properties of human adult and fetal multipotent mesenchymal stem cells,” Journal of Biomedical Science, vol. 18, article 49, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. J. Hipp and A. Atala, “Sources of stem cells for regenerative medicine,” Stem Cell Reviews, vol. 4, no. 1, pp. 3–11, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Hoehn and D. Salk, “Morphological and biochemical heterogeneity of amniotic fluid cells in culture,” Methods in Cell Biology, vol. 26, pp. 11–34, 1982. View at Google Scholar
  12. M. P. Dobreva, P. N. G. Pereira, J. Deprest, and A. Zwijsen, “On the origin of amniotic stem cells: of mice and men,” International Journal of Developmental Biology, vol. 54, no. 5, pp. 761–777, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. M. A. Underwood, W. M. Gilbert, and M. P. Sherman, “Amniotic fluid: not just fetal urine anymore,” Journal of Perinatology, vol. 25, no. 5, pp. 341–348, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. D. C. Kirouac and P. W. Zandstra, “The systematic production of cells for cell therapies,” Cell Stem Cell, vol. 3, no. 4, pp. 369–381, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. S. R. Burger, “Current regulatory issues in cell and tissue therapy,” Cytotherapy, vol. 5, no. 4, pp. 289–298, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. D. G. Halme and D. A. Kessler, “FDA regulation of stem-cell-based therapies,” The New England Journal of Medicine, vol. 355, no. 16, pp. 1730–1735, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. A. J. Friedenstein, R. K. Chailakhjan, and K. S. Lalykina, “The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells,” Cell and Tissue Kinetics, vol. 3, no. 4, pp. 393–403, 1970. View at Google Scholar · View at Scopus
  18. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. E. M. Horwitz, K. Le Blanc, M. Dominici et al., “Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement,” Cytotherapy, vol. 7, no. 5, pp. 393–395, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Zhang, H. Geng, H. Xie et al., “The heterogeneity of cell subtypes from a primary culture of human amniotic fluid,” Cellular and Molecular Biology Letters, vol. 15, no. 3, pp. 424–439, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. P. Bossolasco, T. Montemurro, L. Cova et al., “Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential,” Cell Research, vol. 16, no. 4, pp. 329–336, 2006. View at Publisher · View at Google Scholar · View at Scopus
  22. S. Arnhold, S. Glüer, K. Hartmann et al., “Amniotic-fluid stem cells: growth dynamics and differentiation potential after a CD-117-based selection procedure,” Stem Cells International, vol. 2011, Article ID 715341, 12 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. M. G. Roubelakis, K. I. Pappa, V. Bitsika et al., “Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: comparison to bone marrow mesenchymal stem cells,” Stem Cells & Development, vol. 16, no. 6, pp. 931–951, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. M. S. Tsai, S. M. Hwang, Y. L. Tsai, F. C. Cheng, J. L. Lee, and Y. J. Chang, “Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells,” Biology of Reproduction, vol. 74, no. 3, pp. 545–551, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Lindenmair, S. Wolbank, G. Stadler et al., “Osteogenic differentiation of intact human amniotic membrane,” Biomaterials, vol. 31, no. 33, pp. 8659–8665, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. N. Zakaria, C. Koppen, V. Van Tendeloo, Z. Berneman, A. Hopkinson, and M. J. Tassignon, “Standardized limbal epithelial stem cell graft generation and transplantation,” Tissue Engineering—Part C, vol. 16, no. 5, pp. 921–927, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. J. P. Thiery, H. Acloque, R. Y. J. Huang, and M. A. Nieto, “Epithelial-mesenchymal transitions in development and disease,” Cell, vol. 139, no. 5, pp. 871–890, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. M. A. Nieto, “The ins and outs of the epithelial to mesenchymal transition in health and disease,” Annual Review of Cell and Developmental Biology, vol. 27, pp. 347–376, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. P. De Coppi, G. Bartsch, M. M. Siddiqui et al., “Isolation of amniotic stem cell lines with potential for therapy,” Nature Biotechnology, vol. 25, no. 1, pp. 100–106, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. T. Phermthai, Y. Odglun, S. Julavijitphong et al., “A novel method to derive amniotic fluid stem cells for therapeutic purposes,” BMC Cell Biology, vol. 11, article 79, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Crisan, S. Yap, L. Casteilla et al., “A perivascular origin for mesenchymal stem cells in multiple human organs,” Cell Stem Cell, vol. 3, no. 3, pp. 301–313, 2008. View at Publisher · View at Google Scholar · View at Scopus
  33. M. E. Wall, S. H. Bernacki, and E. G. Loboa, “Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells,” Tissue Engineering, vol. 13, no. 6, pp. 1291–1298, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. A. Jezierski, A. Gruslin, R. Tremblay et al., “Probing stemness and neural commitment in human amniotic fluid cells,” Stem Cell Reviews and Reports, vol. 6, no. 2, pp. 199–214, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. M. G. Roubelakis, V. Bitsika, D. Zagoura et al., “In vitro and in vivo properties of distinct populations of amniotic fluid mesenchymal progenitor cells,” Journal of Cellular and Molecular Medicine, vol. 15, no. 9, pp. 1896–1913, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. P. Mafi, S. Hindocha, R. Mafi, M. Griffin, and W. S. Khan, “Adult mesenchymal stem cells and cell surface characterization—a systematic review of the literature,” The Open Orthopaedics Journal, vol. 5, pp. 253–260.
  37. V. Karantza, “Keratins in health and cancer: more than mere epithelial cell markers,” Oncogene, vol. 30, no. 2, pp. 127–138, 2011. View at Publisher · View at Google Scholar · View at Scopus
  38. P. Singh, C. Carraher, and J. E. Schwarzbauer, “Assembly of fibronectin extracellular matrix,” Annual Review of Cell and Developmental Biology, vol. 26, pp. 397–419, 2010. View at Publisher · View at Google Scholar · View at Scopus
  39. H. Oda and M. Takeichi, “Structural and functional diversity of Cadherin at the adherens junction,” Journal of Cell Biology, vol. 193, no. 7, pp. 1137–1146, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. J. D. Klein, C. G. B. Turner, A. Ahmed, S. A. Steigman, D. Zurakowski, and D. O. Fauza, “Chest wall repair with engineered fetal bone grafts: an efficacy analysis in an autologous leporine model,” Journal of Pediatric Surgery, vol. 45, no. 6, pp. 1354–1360, 2010. View at Publisher · View at Google Scholar · View at Scopus
  41. J. Kim, Y. Lee, H. Kim et al., “Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells,” Cell Proliferation, vol. 40, no. 1, pp. 75–90, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. P. S. In 't Anker, S. A. Scherjon, C. Kleijburg-van der Keur et al., “Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation,” Blood, vol. 102, no. 4, pp. 1548–1549, 2003. View at Publisher · View at Google Scholar · View at Scopus
  43. M. S. Tsai, S. M. Hwang, K. D. Chen et al., “Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow,” Stem Cells, vol. 25, no. 10, pp. 2511–2523, 2007. View at Publisher · View at Google Scholar · View at Scopus
  44. Y. B. Zheng, Z. L. Gao, C. Xie et al., “Characterization and hepatogenic differentiation of mesenchymal stem cells from human amniotic fluid and human bone marrow: a comparative study,” Cell Biology International, vol. 32, no. 11, pp. 1439–1448, 2008. View at Publisher · View at Google Scholar · View at Scopus
  45. H. Herrmann, H. Bär, L. Kreplak, S. V. Strelkov, and U. Aebi, “Intermediate filaments: from cell architecture to nanomechanics,” Nature Reviews Molecular Cell Biology, vol. 8, no. 7, pp. 562–573, 2007. View at Publisher · View at Google Scholar · View at Scopus
  46. L. H. Gu and P. A. Coulombe, “Keratin function in skin epithelia: a broadening palette with surprising shades,” Current Opinion in Cell Biology, vol. 19, no. 1, pp. 13–23, 2007. View at Publisher · View at Google Scholar · View at Scopus
  47. P. De Coppi, A. Callegari, A. Chiavegato et al., “Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells,” Journal of Urology, vol. 177, no. 1, pp. 369–376, 2007. View at Publisher · View at Google Scholar · View at Scopus
  48. M. Orciani, C. Morabito, M. Emanuelli et al., “Neurogenic potential of mesenchymal-like stem cells from human amniotic fluid: the influence of extracellular growth factors,” Journal of Biological Regulators and Homeostatic Agents, vol. 25, no. 1, pp. 115–130, 2011. View at Google Scholar · View at Scopus
  49. O. Parolini, F. Alviano, G. P. Bagnara et al., “Concise review: isolation and characterization of cells from human term placenta: outcome of the First International Workshop on Placenta Derived Stem Cells,” Stem Cells, vol. 26, no. 2, pp. 300–311, 2008. View at Publisher · View at Google Scholar · View at Scopus
  50. S. A. Mani, W. Guo, M. J. Liao et al., “The epithelial-mesenchymal transition generates cells with properties of stem cells,” Cell, vol. 133, no. 4, pp. 704–715, 2008. View at Publisher · View at Google Scholar · View at Scopus
  51. V. L. Battula, K. W. Evans, B. G. Hollier et al., “Epithelial-mesenchymal transition-derived cells exhibit multilineage differentiation potential similar to mesenchymal stem cells,” Stem Cells, vol. 28, no. 8, pp. 1435–1445, 2010. View at Publisher · View at Google Scholar · View at Scopus
  52. S. F. Badylak, D. Taylor, and K. Uygun, “Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds,” Annual Review of Biomedical Engineering, vol. 13, pp. 27–53, 2011. View at Publisher · View at Google Scholar · View at Scopus
  53. J. J. Song and H. C. Ott, “Organ engineering based on decellularized matrix scaffolds,” Trends in Molecular Medicine, vol. 17, no. 8, pp. 424–432, 2011. View at Publisher · View at Google Scholar · View at Scopus
  54. T. W. Gilbert, T. L. Sellaro, and S. F. Badylak, “Decellularization of tissues and organs,” Biomaterials, vol. 27, no. 19, pp. 3675–3683, 2006. View at Publisher · View at Google Scholar · View at Scopus
  55. N. F. Davis, B. B. McGuire, A. Callanan, H. D. Flood, and T. M. McGloughlin, “Xenogenic extracellular matrices as potential biomaterials for interposition grafting in urological surgery,” Journal of Urology, vol. 184, no. 6, pp. 2246–2253, 2010. View at Publisher · View at Google Scholar · View at Scopus
  56. P. M. Crapo, T. W. Gilbert, and S. F. Badylak, “An overview of tissue and whole organ decellularization processes,” Biomaterials, vol. 32, no. 12, pp. 3233–3243, 2011. View at Publisher · View at Google Scholar · View at Scopus
  57. T. Shinoka and C. Breuer, “Tissue-engineered blood vessels in pediatric cardiac surgery,” Yale Journal of Biology and Medicine, vol. 81, no. 4, pp. 161–166, 2008. View at Google Scholar · View at Scopus