Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions

<table>(A) Characterization and differentiation of iPSC colonies generated on xeno-free conditions. Colonies were stained for pluripotent markers: NANOG (b), SSEA4 (c), SOX2 (e), Tra1-60 (f), and phase ((a) and (d)). (B) These colonies were differentiated <i>in vitro</i> into embryoid bodies (EBs) comprising of the three embryonic germ layers: endoderm marker <i>α</i> fetoprotein (AFP) (g), mesodermal marker smooth muscle actin (SMA) (h), and ectoderm marker Beta 3-tubulin (Beta III) (i).</table>

Stem Cells International

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Figure 3

Figure 3: Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions 