Research Article

Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

Figure 8

Cx43 expression in the control and injured motor cortices. Immunohistochemistry of Cx43 (green) in the brains of mice subjected to sham surgery (a,c,e) and motor cortex lesion (b, d, f, g). (a)-(b) The stitched confocal images show the expression of Cx43 in the control (a) and injured (b) brains. (c)-(d). Higher magnification images of the areas of motor cortex indicated by insets in (a) and (b) are shown in (c) (sham) and d (lesion). Increased Cx43 staining was observed in the cortex adjacent to the injury site compared to sham cortex. (e)–(g). Double-labeling with GFAP (red) indicated that Cx43 (green) was expressed mainly in astrocytes (red), and more abundant in the injured cortex (f, g), compared to sham cortex (e). Nuclei were stained with Hoechst (blue). All the sections were coronal. Scale bar: 1000 μm (a)-(b), 100 μm (c, d, g); 10 μm (e, f).
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