Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

Table 1

Sequence and annealing temperatures for RT-PCR.

Designation	Sequence (5′-3′)	Annealing temp. (°C)	Amplicon size (bp)	Ref.
CX26-F	CTGCAGCTGATCTTCGTGTC	55	308	[18]
CX26-R	AAGCAGTCCACAGTGTTG
CX30-F	GCTACCTGCTGCTGAAAGTG	58	326	[40]
CX30-R	CGTTGTGTATGAATGGAGCA
CX32-F	GACAGGTTTGTACACCTTGC	58	500	[41]
CX32-R	CGTCGCACTTGACCAGCCGC
CX36-F	AACGCCGCTACTCTACAGTCTTCC	55	268	[20]
CX36-R	GATGCCTTCCTGCCTTCTGAGCTT
CX37-F	GTTGCTGGACCAGGTCCAGG	58	416	[40]
CX37-R	GGATGCGCAGGCGACCATCT
CX40-F	GTACACAAGCACTCGACCGT	58	509	[40]
CX40-R	GCAGGGTGGTCAGGAAGATT
CX43-F	CAATCACTTGGCGTGACTTC	58	408	[40]
CX43-R	GTTTGGGCAACCTTGAGTTC
CX45-F	GGAGCTTCCTGACTCGCCTGC	58	467	[40]
CX45-R	CGGCCATCATGCTTAGGTTT
GAPDH-F	CATGACCACAGTCCATGCCATCACT	58	461
GAPDH-R	TGAGGTCCACCACCCTGTTGCTGTA