Development of a Functional Schwann Cell Phenotype from Autologous Porcine Bone Marrow Mononuclear Cells for Nerve Repair

Figure 1

Series of light micrographs showing that porcine BM-MNCs failed to thrive in the chemical induction media. The BM-MNCs were initially plated in 𝛼-MEM, 2 mM L-glutamine, and 10% FBS media for 48 hours (a). Cultures were then treated with 1 mM 𝛽-mercaptoethanol added for 24 hours (b); (c) represents cultured cells in 𝛼-MEM media for four days after being treated for 24 hours in 𝛽-mercaptoethanol. Scale bar, 250 𝜇m.