Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency

Table 1

Common techniques to form human embryoid bodies.

	Controls EB size	Need for single cell suspension	Large-scale bioreactor production	Need for special equipment	References
Heterogeneous methods
Liquid suspension culture	No	No	No	No	[15, 16]
Stirred flask culture	No	No	Yes	Yes	[27]
Rotary cell culture systems (RCCSS)	No	No	Yes	Yes	[28, 29]
Homogeneous methods
Hanging drop culture	Yes	Yes	No	No	[13, 30, 31]
Low adhesion U-bottom multiwell plates	Yes	No	No	No	[14]
Indented solid microsurfaces (Aggrewell^TM)	Yes	Yes	No	Yes	[32]
Other methods
Hydrogels (e.g., methylcellulose, agarose, alginate)	No	Yes	No	No	[15, 33, 34]