Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency

Table 2

Typical markers for the analysis of human embryoid bodies.

	Marker name	Alternate name	Marker type	Suitable marker for gene expression	Suitable marker for IHC	Reference
Pluripotency
	Oct4 (POU5F1)	Octamer-binding transcription factor 4	Transcription factor	Yes	Yes	[44]
	Nanog	n/a	Transcription factor	Yes	Yes	[45]
	REX-1	Zinc finger protein 42 homolog (ZFP42)	Transcription factor	Yes	Yes	[2]
	SOX-2	SRY (sex determining region Y)-box 2	Transcription factor	Yes	Yes	[46]
	SSEA-3	Stage-specific embryonic antigen 3 (SSEA-3)	Surface antigen	No	Yes	[47]
	SSEA-4	Stage-specific embryonic antigen 3 (SSEA-4)	Surface antigen	No	Yes	[47]
	Tra-I-60	n/a	Surface antigen	No	Yes	[48]
	Tra-I-81	n/a	Surface antigen	No	Yes	[48]
Ectoderm
	GFAP	Glial fibrillary acidic protein	Glial intermediate filament	Yes	Yes	[49]
	Nestin	n/a	Intermediate filament	Yes	Yes	[49]
	Pax-6	Paired box gene 6	Transcription factor	Yes	Yes	[50]
	Sox-1	Sex determining region Y-box 1	Transcription factor	Yes	Yes	[51]
Endoderm
	AFP	Alpha-fetoprotein	Plasma Protein	Yes	No	[52]
	Amylase	Alpha-amylase	Metabolic Enzyme	Yes	Yes	[53]
	FOXA2	Hepatocyte nuclear factor 3-beta (HNF-3B)	Transcription Factor	Yes	Yes	[54]
	PDX1	Pancreatic and duodenal homeobox 1, insulin promoter factor 1	Transcription factor	Yes	Yes	[55]
	GATA4	GATA binding protein 4	Transcription factor	Yes	Yes	[56]
Mesoderm
	Brachyury	n/a	Transcription factor	Yes	Yes	[57]
	CD34	Cluster of differentiation molecule 34	Surface antigen	No	Yes	[58]
	FLT1	Vascular endothelial growth factor receptor 1	Surface receptor	Yes	Yes	[59]
	RUNX1	AML1, Runt-related transcription factor 1	Transcription factor	Yes	Yes	[60]