Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

Figure 3

Proliferation in long-term propagated porcine ventral mesencephalic (VM) neural tissue spheres (NTS) visualized by Ki67 and BrdU immunostaining of histological sections. Photomicrographs of NTS showing expression of Ki67 at (a) 63 days in vitro (DIV) (passage (P) 6), (b) 141 DIV (P12), (c) 237 DIV (P18) and BrdU labelling at (d) 63 DIV, (e) 141 DIV, and (f) 237 DIV. Scale bars: 100 μm (overview) and 20 μm (insert). Quantification of Ki67 (g) and BrdU (h) expressing cells showed significantly more Ki67-positive cells at P18 as compared to P6, whereas there were significantly more BrdU-positive cells at P6 and P12 as compared to P18. Data are expressed as mean ± SEM (𝑛=15–19; *𝑃<0.05, ***𝑃<0.001).

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