Research Article

Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

Table 1

Differentiation of long-term propagated neural tissue-spheres (NTS) derived from the embryonic porcine ventral mesencephalon (VM).

Age (DIV)Passage (P)Protocol(s)Duration (days)

51(1) Mitogen-free
P2(2) a-FGF, forskolin, TPA, db-cAMP 25
170(1) Mitogen-free
P14(2) a-FGF, forskolin, TPA, db-cAMP 10
232(1) Mitogen-free
P18(2) FGF8 + sonic hedgehog 10