Review Article

Nonhuman Primate Induced Pluripotent Stem Cells in Regenerative Medicine

Figure 2

Retroviral reprogramming vector designed to deliver four reprogramming factors (Oct4, Sox2, Klf4, and c-Myc; OSKM) in a single virus using “self-cleaving” peptides, which support efficient polycistronic expression from a single promoter [8]. In this version, expression is driven by the 5′ LTR. Additionally, loxP sites are present just before and just after the OSKM coding region, enabling excision of the vector from the genome of the reprogrammed cells. This vector was constructed by replacing the internal promoter (P) and eukaryotic selection marker of retroviral vector pLXSN by the OSKM sequence from FUW-OSKM [8].
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