Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Figure 1
Stem cell colony size was controlled by section size using laser-mediated passage. (a) Brightfield images of human iPSC (top, middle) and ESC (bottom) cultures cut into 100–250 μm sections. Scale bar, 1 mm. (b) Colony size over time following propagation of 100–250 μm iPSC sections (top) or ESC sections (bottom) by laser-mediated passage. Number of cells per colony were manually counted using Hoechst stained cultures (left, colonies per data point). Longest diameter of each colony was manually measured using brightfield images (right, colonies per data point). Data are shown as mean + s.d.
