Comparison of the Direct Effects of Human Adipose- and Bone-Marrow-Derived Stem Cells on Postischemic Cardiomyoblasts in an In Vitro Simulated Ischemia-Reperfusion Model
Figure 1
Ischemia-reperfusion model. (a) Representative fluorescent microscopic picture showing H9c2 cells injured with our ischemia-reperfusion model and treated with Vybrant DiD (ex/em: 644/665 nm, blue) labeled cells. The cytoplasm of the living cells is stained with calcein-AM (ex/em: 494/517 nm, green), the nuclei of the necrotic cells are ethidium homodimer-2 stained (ex/em: 536/624 nm, red). (b) Flow cytometric histogram on the distinction between stem cells and H9c2 cells based on DiD staining. (c) Schematic representation of the experimental protocol.
