Research Article

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Figure 2

Activation of mesenchymal stem cell markers. The activation of Cd146 (~479 bp) (a) and Cd166 (~630 bp) (b) was observed only in cells before differentiation, indicating that the cells were mesenchymal stem cells. Cd31 was inactivated before and after neuronal differentiation (c). Gapdh (~717 bp), a housekeeping gene, was expressed before and after differentiation (d). Panel (i) is representing undifferentiated cells, that is, cells before neuronal differentiation, while panel (ii) is representing differentiated cells, that is, cells after neuronal differentiation.
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(a)
250740.fig.002b
(b)
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(c)
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(d)