Research Article

Differentiation of Dental Pulp Stem Cells into Neuron-Like Cells in Serum-Free Medium

Figure 3

Characteristics of differentiated cells. Neuron-like cells appeared among the dental pulp stem cells ((a), (b)) 5 days after neuronal induction (c). The perikaryon, dendrites, and axons of neurons are indicated, respectively, by white arrows, open arrowheads, and black arrows (d). Immunofluorescence staining for neuron markers MAP2 was performed after 5 days of neuronal induction ((e), (f)). MAP2 marker was not detected in DPSCs without neuronal induction, that is, negative control (g).
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(a)
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(b)
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(c)
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(d)
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(e)
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(f)
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(g)