Immunomodulatory Effects of Mesenchymal Stromal Cells Revisited in the Context of Inflammatory Cardiomyopathy
Figure 1
Cardiac-derived cells reduce T-cell proliferation in a cell-contact-dependent manner involving the receptor B7 H-1. (a) Expression of B7-H1 on CardAPs after specific siRNA knockdown. Red coloured histogram represents isotype control, the orange line indicates the expression of B7-H1 on CardAPs under basal conditions. The light blue line represents B7-H1 expression after exogenous stimulation with 5 ng/ml of human IFN-. The green line shows absence of B7-H1 expression after its specific siRNA knockdown, which even upon IFN- treatment was not restored (dark blue line). (b) Bar graphs represent the mean ± SEM of B7-H1 positive CardAPs or CardAPs after specific siRNA knockdown of B7-H1, as indicated, depicted as the % of gated CardAPs, under basal culture conditions (open bars) and after 72 h treatment with human IFN- (closed bars) with /group. (c) The immunosuppressive function of CardAPs is mediated via B7-H1. Spleen MNCs from control (open bars) and CVB3-infected (closed bars) mice were activated and labeled with 10 μM of CFSE to be able to measure cell proliferation, and cultured for 48 h in the absence or presence of CardAPs or CardAPs of which B7-H1 was knocked down, followed by flow cytometry and analysis with FlowJo 8.7. software. Bar graphs represent the division index of spleen MNCs from control and CVB3-infected mice co-cultured for 48 h in the absence or presence of CardAPs or CardAPs of which B7-H1 was knocked down, as indicated, with /group.
